OESTRADIOL 6-(O-CARBOXYMETHYL)OXIME:BSA FITC CONJUGATE
Flammability | 1 | |
Toxicity | 2 | |
Body Contact | 2 | |
Reactivity | 2 | |
Chronic | 2 | |
SCALE: Min/Nil=0 Low=1 Moderate=2 High=3 Extreme=4 |
Reagent. Oestradiol is used in replacement therapies in deficiency states, for the
treatment of conditions such as primary amenorrhoea, delayed onset of puberty, and the
management of menopausal syndrome. Also used for the treatment of malignant neoplasms of
the prostate and of the breast of menopausal women. Oestradiol is administered as tablets,
implants, by intramuscular injection or topically. Oestradiol is the most active of the
naturally occurring oestrogenic hormones formed in the ovarian follicles under the
influence of the pituitary. The hormone controls the development and maintenance of female
sex organs, secondary sex characteristics, the mammary glands, functions of the uterus and
accessory organs, and the cyclic changes in the cervix and vagina. Low physiological
amounts stimulate the gonadotrophic and lactogenic activities of the anterior pituitary
whilst large amounts are inhibitory. In late pregnancy oestradiol increases the
spontaneous activity of the uterine muscle and its response to oxytocic drugs.
"beta-estradiol 6-(O-carboxymethyloxime):BSA (protein) fluoresceinisothiocyanate
conjugate", "beta-estradiol 6-(O-carboxymethyloxime):BSA (protein)
fluoresceinisothiocyanate conjugate", "1, 3, 5-estratriene-3, 17beta-diol 6-(O-
carboxymethyloxime):BSA FITC", "1, 3, 5-estratriene-3, 17beta-diol 6-(O-
carboxymethyloxime):BSA FITC", "sex hormone/ oestrogen/ estrogen steroid"
May form explosive peroxides.
Although ingestion is not thought to produce harmful effects, the material may still be damaging to the health of the individual following ingestion, especially where pre- existing organ (e.g. liver, kidney) damage is evident. Present definitions of harmful or toxic substances are generally based on doses producing mortality (death) rather than those producing morbidity (disease, ill-health). Gastrointestinal tract discomfort may produce nausea and vomiting. In an occupational setting however, ingestion of insignificant quantities is not thought to be cause for concern. The estrogens may produce dose-related nausea and vomiting, undesirable uterine growth, proliferation and withdrawal bleeding or loss of periods. It causes enlargement of the breasts in males. Other side effects include weight gain, swelling, breast tenderness, liver dysfunction, jaundice, depression, headache, and dizziness. Growth may be stunted due to premature closing of the growth plates. Skin reactions can include excess pigmentation of the face, rashes, and hives. Redness, itching and blistering has also been reported.
Although the material is not thought to be an irritant, direct contact with the eye may produce transient discomfort characterized by tearing or conjunctival redness (as with windburn). The dust may produce eye discomfort causing smarting, pain and redness.
Skin contact with the material may damage the health of the individual; systemic effects may result following absorption. The material is not thought to be a skin irritant (as classified using animal models). Temporary discomfort, however, may result from prolonged dermal exposures. Good hygiene practice requires that exposure be kept to a minimum and that suitable gloves be used in an occupational setting. Toxic effects may result from skin absorption. Sensitization may result in allergic dermatitis responses includingrash, itching, hives or swelling of extremities.
The material is not thought to produce adverse health effects or irritation of the respiratory tract (as classified using animal models). Nevertheless, good hygiene practice requires that exposure be kept to a minimum and that suitable control measures be used in an occupational setting. Persons with impaired respiratory function, airway diseases and conditions such as emphysema or chronic bronchitis, may incur further disability if excessive concentrations of particulate are inhaled. Respiratory sensitization may result in allergic/asthma like responses; from coughing and minor breathing difficulties to bronchitis with wheezing, gasping.
There is some evidence that inhaling this product is more likely to cause a sensitization reaction in some persons compared to the general population.
Principal routes of exposure are by accidental skin and eye contact andinhalation of generated dusts. When administered orally to female mice, oestradiol induced increased adenocarcinomas of the mammary gland, cervix and uterus, and osteosarcomas of the cranium and adenoacanthomas of the uterus. Subcutaneous or intramuscular injection induced increased incidences of lymphosarcomas in mice of both sexes. Subcutaneous implants induced mammary tumours, including adenocarcinomas, papillary carcinomas and anaplastic carcinomas in adult and newborn male and female mice and female rats; pituitary chromophobe adenomas in male rats, fibromyomas of the uterus, mesentary and abdomen in female guinea pigs; and malignant tumours in hamsters of both sexes. Long term administration of estrogens can greatly increase the risk of endometrial cancer, especially after menopause. Males exposed can develop enlarged breasts and other feminizing effects, nipple pigmentation, withering of testicles, sterility, impotence and altered distribution of hair. Females exposed can develop breast enlargement and menstrual disorders and other effects on the reproductive system. Children born to exposed mothers can show breast enlargement in boys and early puberty in girls. Children who are themselves exposed may develop increased rate of bone maturation (leading to reduced final stature), strong pigmentation of the sexual organs and feminizing syndrome. Exposure before birth may be associated with limb defects and congenital heart deformities. Repeated swallowing can cause nausea, vomiting, abdominal cramps, loss of appetite, bowel inflammation, headache, dizziness, irritability, depression, general unwellness, involuntary jerky movements and convulsions. Swelling, weight change, increased blood pressure and risk of clotting, liver abnormalities, uremia have all been reported. Long-term users may also show an increased risk of developing gallstones, increased blood fats, acute pancreas inflammation and aggravation of porphyria. The eye may develop damage, increased corneal curvature with contact lens intolerance. Skin effects include itching, hives, inflammation, increased pigmentation, sensitivity to light, loss of scalp hair and hairiness. Allergic reactions include a red rash and jaundice. Susceptibility to Candida infections and changes to sex drive may occur. Application of estrogen-containing cream had produced breast enlargement. Dusts produced by proteins can sometimes sensitize workers like other foreign bodies. Symptoms include asthma appearing soon after exposure, with wheezing, narrowing of the airways and breathing difficulties. There may also be a chronic cough, phlegm, fever, muscle pains, fatigue and airway obstruction; chest X-rays may show a characteristic net- like pattern or scarring at the tip and base. There may also be chest discomfort, headache, stomachache and a general feeling of unwellness. Often the clinical picture is similar to "farmer's lung" and other allergic lung inflammations. Prolonged contact with the skin can cause pain, redness, inflammation and ulceration. Repeated attacks can cause loss of lung function due to scarring. Exposure to small quantities may induce hypersensitivity reactions characterized by acute bronchospasm, hives (urticaria), deep dermal wheals (angioneurotic edema), running nose (rhinitis) and blurred vision . Anaphylactic shock and skin rash (non-thrombocytopenic purpura) may occur. An individual may be predisposed to such anti-body mediated reaction if other chemical agents have caused prior sensitization (cross-sensitivity). Isothiocyanates have been used as inducers of apoptosis (programmed cell death). Isothiocyanate-induced apoptosis may suppress the growth of pre-clinical tumours and contribute to the well-established decreased cancer incidence associated with a vegetable- rich diet. Under physiological conditions, isothiocyanates react rapidly with cysteinyl thiol groups to form S-thiocarbamoylcysteine derivatives - which is reversible, and slowly with amino groups to form thioureas. A competing reaction is the hydrolysis of isothiocyanates to corresponding monothiocarbamates and fragmentation to the related amines. The chemopreventive activity of dietary isothiocyanates has been attributed to the decreased activation and increased conjugation of excretion of carcinogens. Isothiocyanates and their cysteine conjugates inhibited cytochrome P450 isozymes 1A1, 1A2, 2B1 and 2E1. They also induced increased activities of enzymes involved in the detoxification and conjugation of carcinogens for elimination: glutathione S-transferases, quinone reductase, epoxide hydrolase and UDP glucuronosyl transferases. A further feature of the pharmacological activity of dietary isothiocyanates and related S-(N- thiocarbamoyl)cysteine derivatives was their anticancer activity. Inhibition of tumour growth in pre-clinical development by isothiocyanates may contribute to the association of decreased cancer incidence with dietary glucosinolate consumption. Cruciferous vegetables are the major dietary source of naturally occurring thioglucosides or glucosinolates. Glucosinolates are degraded non-enzymatically and enzymatically during food preparation, cooking and chewing.